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antibodies against flag  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc antibodies against flag
    Antibodies Against Flag, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 855 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+flag+antibody/DYKDDDDK+Tag+Mouse+mAb/pm42811469-268-1-14
    Average 96 stars, based on 855 article reviews
    antibodies against flag - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Incubation:

    Article Title: Whirly Transcription Factor NtWHY1 Positively Regulates the Biosynthesis of Cembranoid Diterpenoids by Directly Targeting NtCBTS in Tobacco.
    Article Snippet: .. A mixture of DNA and NtWHY1- FLAG protein was incubated with protein A agarose beads and mouse anti- FLAG antibody (#14793, Cell Signaling Technology) at 4°C overnight. ..

    Article Title: Basal interaction of the orphan receptor GPR101 with arrestins leads to constitutive internalization.
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Article Title: The nuclear transportation of CHRONO regulates the circadian rhythm
    Article Snippet: After 48 h, cells were lysed in Western and IP cell lysis buffer (#P0013, Beyotime, Shanghai, China) at 4 °C for 30 min. .. The supernatant of the lysate was prepared after centrifugation at 4 °C, 12,000 rpm for 20 min and immunoprecipitated with mouse anti-Myc antibody or mouse anti-Flag antibody and mouse immunoglobulin G with gentle rotation at 4 °C overnight, then incubated with the Protein G magnetic beads (#9006S, Cell Signaling Technology) solution at 4 °C for 2 h. The beads were washed with pre-cold PBS for five times with gentle rotation. ..

    Article Title: Regulation of BRCA1 stability through the tandem UBX domains of isoleucyl-tRNA synthetase 1
    Article Snippet: .. Cells were blocked in blocking buffer (PBS containing 3% BSA) for 1 hr and then incubated with a mouse anti-Flag antibody (8146S, Cell Signaling, 1:250) diluted in blocking buffer for 2 hr at RT. .. After four washes, cells were incubated for 1 hr with a goat anti-mouse Alexa Fluor 488-conjugated secondary antibody (Invitrogen) diluted in blocking buffer.

    Staining:

    Article Title: Amphioxus tyrosine kinase SYK inhibits NF-κB activation through MyD88-dependent mechanism.
    Article Snippet: Approximately 24 h after the transfection, medium was removed, and the cells were wash with phosphate-buffered saline (PBS) for three times, fixed with pre-cold acetone, and permeabilized with 0.1% Triton X-100 (P0096, Beyotime). .. The cells were blocked for 1 h at room temperature with 5% goat serum and then stained with rabbit anti-HA antibody (3724, Cell Signaling Technology) and mouse anti-FLAG antibody (8146, Cell Signaling Technology) at 4°C overnight. .. After washed for three times with PBS, the cells were stained with Alexa Fluor 488-conjuated goat anti-rabbit IgG (ab150077, Abcam) and Alexa Fluor 555-conjuated goat anti-mouse IgG antibodies (ab150078, Abcam) and counterstained with DAPI (C1002, Beyotime).

    Western Blot:

    Article Title: Interferon-induced MXB protein restricts vimentin-dependent viral infection
    Article Snippet: .. The antibodies used for WB included rabbit anti-VIM antibody (ab92547, Abcam), mouse anti-VIM antibody (ab8978, Abcam), rabbit anti-VIM S83 antibody (AP0799, ABclonal), rabbit anti-VIM S82 antibody (ab52943, Abcam), rabbit anti-VIM S72 antibody (ab52944, Abcam), rabbit anti-VIM S55 antibody (ab217673, Abcam), rabbit anti-VIM S38 antibody (ab52942, Abcam), rabbit anti-AKT antibody (ab8805, Abcam), rabbit anti-Flag antibody (D6W5B, CST), mouse anti-Flag antibody (9A3, CST), goat anti-Flag antibody (ab1257, Abcam), rabbit anti-HA antibody (C29F4, CST), rabbit anti-MXB antibody (A83265, NOVUS), mouse anti-actin antibody (AC004, ABclonal), mouse anti-PDI antibody (ab2792, Abcam), rabbit anti-p84 antibody (ab131268, Abcam), rabbit anti-CYPA antibody (ab41684, Abcam), mouse anti-HCV core antibody (ab2740, Abcam), and rabbit anti-HIV-1 p24 antibody (ab9071, Abcam). .. After probing with specific antibodies, the membrane was further incubated with secondary antibodies, including HRP-conjugated goat anti-mouse (ZB2305, ZSGB-Bio), goat anti-rabbit (ZB2301, ZSGB-Bio), or rabbit anti-goat (ZB2306, ZSGB-Bio).

    Cell-Signaling:

    Article Title: Basal interaction of the orphan receptor GPR101 with arrestins leads to constitutive internalization.
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    other:

    Article Title: Regulation of STING activation by phosphoinositide and cholesterol
    Article Snippet: The following antibodies were used: rabbit anti-STING antibody (Cat. #13647S), rabbit anti-phospho-STING (S366) antibody (Cat. #19781S), rabbit anti-phospho-TBK1/NAK(S172) antibody (Cat. #5483S), mouse anti-flag antibody (Cat. #8146S) and mouse anti-α-Tubulin antibody (Cat. #3873S) were purchased from Cell signaling Technology; rabbit anti-phospho-IRF3(S386) (Cat. #ab76493) was purchased from Abcam; The secondary antibodies including anti-rabbit immunoglobulin G (IgG) (H+L) (Dylight 800 conjugates, Cat. #5151) and anti-mouse IgG (H+L) (Dylight 680 conjugates, Cat. #5470) were also purchased from Cell Signaling Technology.

    Centrifugation:

    Article Title: The nuclear transportation of CHRONO regulates the circadian rhythm
    Article Snippet: After 48 h, cells were lysed in Western and IP cell lysis buffer (#P0013, Beyotime, Shanghai, China) at 4 °C for 30 min. .. The supernatant of the lysate was prepared after centrifugation at 4 °C, 12,000 rpm for 20 min and immunoprecipitated with mouse anti-Myc antibody or mouse anti-Flag antibody and mouse immunoglobulin G with gentle rotation at 4 °C overnight, then incubated with the Protein G magnetic beads (#9006S, Cell Signaling Technology) solution at 4 °C for 2 h. The beads were washed with pre-cold PBS for five times with gentle rotation. ..

    Immunoprecipitation:

    Article Title: The nuclear transportation of CHRONO regulates the circadian rhythm
    Article Snippet: After 48 h, cells were lysed in Western and IP cell lysis buffer (#P0013, Beyotime, Shanghai, China) at 4 °C for 30 min. .. The supernatant of the lysate was prepared after centrifugation at 4 °C, 12,000 rpm for 20 min and immunoprecipitated with mouse anti-Myc antibody or mouse anti-Flag antibody and mouse immunoglobulin G with gentle rotation at 4 °C overnight, then incubated with the Protein G magnetic beads (#9006S, Cell Signaling Technology) solution at 4 °C for 2 h. The beads were washed with pre-cold PBS for five times with gentle rotation. ..

    Gentle:

    Article Title: The nuclear transportation of CHRONO regulates the circadian rhythm
    Article Snippet: After 48 h, cells were lysed in Western and IP cell lysis buffer (#P0013, Beyotime, Shanghai, China) at 4 °C for 30 min. .. The supernatant of the lysate was prepared after centrifugation at 4 °C, 12,000 rpm for 20 min and immunoprecipitated with mouse anti-Myc antibody or mouse anti-Flag antibody and mouse immunoglobulin G with gentle rotation at 4 °C overnight, then incubated with the Protein G magnetic beads (#9006S, Cell Signaling Technology) solution at 4 °C for 2 h. The beads were washed with pre-cold PBS for five times with gentle rotation. ..

    Magnetic Beads:

    Article Title: The nuclear transportation of CHRONO regulates the circadian rhythm
    Article Snippet: After 48 h, cells were lysed in Western and IP cell lysis buffer (#P0013, Beyotime, Shanghai, China) at 4 °C for 30 min. .. The supernatant of the lysate was prepared after centrifugation at 4 °C, 12,000 rpm for 20 min and immunoprecipitated with mouse anti-Myc antibody or mouse anti-Flag antibody and mouse immunoglobulin G with gentle rotation at 4 °C overnight, then incubated with the Protein G magnetic beads (#9006S, Cell Signaling Technology) solution at 4 °C for 2 h. The beads were washed with pre-cold PBS for five times with gentle rotation. ..

    Blocking Assay:

    Article Title: Regulation of BRCA1 stability through the tandem UBX domains of isoleucyl-tRNA synthetase 1
    Article Snippet: .. Cells were blocked in blocking buffer (PBS containing 3% BSA) for 1 hr and then incubated with a mouse anti-Flag antibody (8146S, Cell Signaling, 1:250) diluted in blocking buffer for 2 hr at RT. .. After four washes, cells were incubated for 1 hr with a goat anti-mouse Alexa Fluor 488-conjugated secondary antibody (Invitrogen) diluted in blocking buffer.



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